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Biotechnology and Biomedical Engineering
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Cell biology Virtual Lab I
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Western Blotting
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Western Blotting
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Western blotting is a technique that .....
Sorts DNA fragement by size
Uses DNA probes to detect DNA fragements
Uses DNA probes to detect RNA fragements
Uses antibodies to detect proteins
2)
Western blotting would be a useful technique to determine expression of a gene in a particular tissue.
True
False
3)
After incubation of membrane with primary antibody, which buffer is used for washing the membrane?
Running buffer
TBS-T buffer
Transfer buffer
SDS buffer
4)
The loading sample containing .......
Sample buffer, protein, and Milli-Q water
Sample buffer and protein
Sample buffer and Milli-Q water
Sample buffer only
5)
The electrophoresis apparatus needs
Electrophoresis tank Buffer solution
Electrophoresis tank
power packs and electrodes
All the above
6)
For verify protein transfer which stain is used?
Ponceau staining
Crystal violet staining
Gram's iodine staining
None of these
7)
Primary antibody is diluted by using ........
5% non -fat dry milk
5% BSA
5% SDS
5% PBS
8)
Secondary antibody is diluted by using......
5% non-fat dry milk
5% BSA
5% SDS
5% PBS
9)
For the detection of protein in membrane which instrument is used?
Flow cytometry
Bio imaging sysyem
UV illuminator
Gel documentation system
10)
Assembly the immunblot sandwitch from cathode to anode. 1. Gel, 2. Filter paper, 3. Sponge, 4. PVDF membrane
2,3,4,1,3,4
3,2,1,4,2,3
1,4,3,2,4,1
4,3,2,1,2,3
Cite this Simulator:
vlab.amrita.edu,. (2012). Western Blotting. Retrieved 24 April 2024, from vlab.amrita.edu/?sub=3&brch=187&sim=1331&cnt=3
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