Assignments

1. Using voltage clamp mode in the simulator with standard ionic concentration calculate the peak current for Na⁺ and K⁺ by varying voltage step value -60 mV to +40 mV with an increment of 10 mV and plot V-I curve by assigning voltage on x-axis and current on y-axis.

2. Calculate the reversal potential and conductance of Na⁺ and K⁺ ion channel from the above plotted V-I curve.

Hint : Draw a straight line on the V-I curve  y-value for x-value ranges from 0 to +50 mV. The point at which the line crosses the x-axis and the voltage at that point is the reversal potential of particular ion. Use Ohm's law to calculate the conductance.

3. Calculate the reversal potential  of Na⁺ ion by plotting V-I curve with standard ionic concentration. Calculate the peak current for Na⁺ and K⁺ by varying voltage step value -60 mV to +40 mV with an increment of 10 mV and apply TTX under "Drugs" tab in the simulator to 100% to block sodium current completely.