Simulation steps: 

         The software can be downloaded from rsbweb.nih.gov/ij/download.html

 Installation and System Requirements

ImageJ is an open source and freely available image analysis software. It can be freely downloaded and installed on any system that has a Java 6 (or later) runtime installed. This includes Linux, Mac OS X and Windows, in both 32-bit and 64-bit modes.

For Windows:

Windows version (both 32-bit and 64-bit) of ImageJ is available bundled with java and without java. If the system is java enabled, the package can be downloaded directly without java. If not the case, it is recommended to download and install the version with java. Double click on the downloaded .exe file to install the software. 

For Linux:

The Linux version of ImageJ (both 32-bit and 64-bit) is available and both versions include java. To install and run the software, extract the downloaded compressed file and execute the ‘run’ script.

For Mac OS X:

ImageJ software for Mac OS X can be downloaded as a compressed file. Expanding the zip file generates a folder “ImageJ” with the executables. The Mac OS X version of the software includes ImageJ64, which uses Java 1.6 in 64-bit mode on Intel Macs running OS X 10.5 or later.

Sample images of bacterial colonies given in the table below. One can select  the image to be analyzed from the provided samples and follow the steps in the Procedure to complete the experiment.

Click on any of the following links, image 1,image 2, image 3 or image 4. The selected image will be displayed on the screen. Right click and follow "Save image as" to save the image on the computer and then analyze the image.

 In case of using amrita virtual lab images for powerpoint presentations, publications, image analysis techniques etc please cite amrita.edu/virtuallabs (Biotechnology and Biomedical Engineering, Biological Image Amalysis Virtual Lab)