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Construction of Maltose Standard Curve by DNS Method
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 Assignment:

 

 

  1. Analyse the protein content in different samples using the above constructed protein standard curve.
  2. Find out the specific activity of beta amylase enzyme.
  3. If 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein when assayed for protein using the Lowry method. What is the concentration of protein in ug/ml in the original sample ?
  4. If you mix two  unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used?
  5. Based on the experimental data provided, estimate the amount of reducing sugar in the given unknown solution by Dinitrosalicylate [DNS] method.    

 


    
 

 

 

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