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Gelatin Zymography




  1.   Practice the gelatin zymography with different samples to check for the presence of gelatinolytic acitivity.
  2.    In which lane do you think does the presence of MMP-13 is observed?       



3.    When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein . What could be  the possible reason for such an observation ?

4. What will happen if the protein that you are assaying using zymography is not fixed properly using Trichloroacetic acid ?

5.Which step of the zymography is crucial for obtaining clear white bands on a blue background ?



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