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Isolation of lymphocytes from whole blood
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Assignments

 

  • Can we isolate the lymphocytes from other sources (except whole blood) by this same procedure? Why?

 

  • Name some other anti coagulating agent used for the lymphocyte isolation procedure?

 

  • Explain the importance of  using the reagents used in this lymphocyte isolation procedure?

 

                         a)   Histopaque 1077 medium

 

                        b)      1X PBS Buffer

 

                        c)      Heparinised green top tube

 

                       d)      Freezing medium

 

                       e)  -80°C freezer

 

 

  • Can we isolate lymphocytes from clotted blood? Explain?

 

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