- Can we isolate the lymphocytes from other sources (except whole blood) by this same procedure? Why?
- Name some other anti coagulating agent used for the lymphocyte isolation procedure?
- Explain the importance of using the reagents used in this lymphocyte isolation procedure?
a) Histopaque 1077 medium
b) 1X PBS Buffer
c) Heparinised green top tube
d) Freezing medium
e) -80°C freezer
- Can we isolate lymphocytes from clotted blood? Explain?