Assignments

• Can we isolate the lymphocytes from other sources (except whole blood) by this same procedure? Why?

• Name some other anti coagulating agent used for the lymphocyte isolation procedure?

• Explain the importance of  using the reagents used in this lymphocyte isolation procedure?

                         a)   Histopaque 1077 medium

                        b)      1X PBS Buffer

                        c)      Heparinised green top tube

                       d)      Freezing medium

                       e)  -80°C freezer

• Can we isolate lymphocytes from clotted blood? Explain?