Immunology Virtual Lab II

The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.

Ouchterlony Double Diffusion -Titration
To introduce the principle of precipitation reactions and differentiate it from agglutination reactions
Ouchterlony Double Diffusion - Patterns
To introduce the principle of precipitation reactions and differentiate it from agglutination reactions
Purification of IgG Antibodies with Ammonium Sulphate
To demonstrate the purification protocols of antibodies for use in research and diagnostics
Removal of Thymus and Spleen from Mice
To understand the concepts of mouse Euthanasia. To learn the basic procedures involved in rodent dissection and how to identify and remove lymphoid organs.
Mouse Anesthesia and Blood Collection
This simulation of Mouse anesthesia and blood collection is to familiarize user to the common anesthesia techniques applied in laboratories on rodents and to introduce basic restraining techniques and blood collection techniques while handling mice.
Parenteral Injections
To understand the basics of handling and manually restraining experimental animals (rodents).
Purification of IgG Antibodies using Affinity Chromatography
Affinity chromatography is unique in purification technique as it offer's high selectivity, high resolution & high capacity for target proteins. It enable the purification of a bio molecule on the basis of it's biological functions or chemic
Fluorescent Labeling of Antibodies
Antibody labeling is an important molecular biological technique.Labeled specific antibodies are widely used in immunoassays to track the protein of interest.Flourescent labeling of antibodies explains the technique to label a protein or antibody with
Fragmentation of IgG Using Papain
To understand the basic principles in fragmentation of IgG to a monovalent Fab fragment using Papain digestion. The cleavage of antibodies using the Proteolytic enzyme is first studied by porter in 1959. IgG is cleaved by Papain to give Fab fragments
Fragmentation of IgG using pepsin
T understand the principles of IgG fragmentation to a divalent F(ab')2 fragment.

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