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Fluorescent Labeling of Antibodies


Student Assignment

  1. What is the importance of labelling the antibodies or proteins?
  2. Explain the steps involved in dialysis of antibodies? 
  3. Calculate the amount of  FITC-reactive dye required to label 2mg/ml of Ig G antibody? 
  4. You are provided with an  antibody  of concentration of 4mg/ml. Show the absorbance of antibody-FITC protein conjugate at 495nm.

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