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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab I
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Bacterial Growth Curve
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Bacterial Growth Curve
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Theory
Procedure
Self Evaluation
Animation
Simulation
Assignment
Reference
Feedback
NPTEL Video
1)
The actively growing and metabolizing phase of the bacterial growth is
Stationary phase
Logarithmic phase
Lag phase
Death phase
2)
The microorganism starts producing inhibitory compounds in stationary phase. This is because
Growth rate and death rate of the microorganisms are equilibrated
Rapid multiplication of organism in the log phase exploited most nutrients; hence the organism is competing for available nutrients
The organism stops its growth
None of the above
3)
An old culture of Escherichia coli is inoculated into a fresh media. The lag phase of the organism is then
Reduced
Remains constant
Increased
None of the above
4)
Optical density is the measure of
Wavelength of light
Transmittance
Width of cuvette
Absorbance
5)
A researcher introduced Staphylococcus aureus into Mannitol salt broth from Nutrient broth. He observed that the organism experience a long lag phase. This is because
Mannitol Salt Broth does not promote the growth of Staphylococcus aureus
The organism is in replicating phase
The organism needs some time to adapt with the new environment
Lack of sufficient inoculum
6)
The growth rate of bacteria is in
Geometric mean
Harmonic mean
Arithmetic mean
Observed mean
7)
The order of the four phases of the growth curve of a bacterial culture are
Lag, Stationary, Exponential, Death
Exponential, Lag, Stationary, Death
Stationary, Lag, Exponential, Death
Lag, Exponential, Stationary, Death
None of the above
8)
The growth rate constant of an exponentially growing population of bacteria is calculated by
generation time divided by ln2
generation time divided by Nt
ln2 divided by the generation time
None of the above
9)
When measuring concentrations using spectrophotometry, the amount of light absorbed is inversely proportional to the concentration of light-absorbing material in the cuvette.
True
False
10)
The generation time of Staphylococcus aureus is
25 minutes
30 minutes
20 minutes
30 seconds
11)
A culture of bacteria produces 5 generations in 2 hours. Calculate the generation time of the bacterium.
30 seconds
24 minutes
1 hour
20 minutes
12)
Starvation proteins are produced during the ............. phase of growth curve
Exponential phase
Death phase
Stationary phase
Lag phase
Cite this Simulator:
vlab.amrita.edu,. (2011). Bacterial Growth Curve. Retrieved 20 April 2024, from vlab.amrita.edu/?sub=3&brch=73&sim=1105&cnt=3
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