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Maintenance and Storage of DH5alpha E.coli cells
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1)Usually glycerol is added at a concentration of 
  

2)Which one is the cryoprotective agent that enters the cell and protects the intracellular environment of the organism? 
  

3)Storage of microorganisms in liquid nitrogen at temperature 
  

4)The cryoprotective agents which are used for lyophilization 
  

5)Which temperature is used in short term preservation methods
  


  


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