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Preparation of Buffer stocks (TBE,TE and TAE)
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Materials required

 

  • Cotton                                                                                                                                                              
  • Spatula                                                                                           
  • magnetic stirrer                                                                       
  • Butter paper   
  • Forceps                                                                              
  • Pipette rack                                                                                     
  • 200ml beaker
  • 1000ml beaker
  • Distilled water
  • measuring cylinders of 100ml , 1000ml(1L) ,  25ml and 10ml
  • Glass rod
  • Weighing balance 
  • pH meter
  • Wash bottle
  • Tissue paper
  • Dropper
  • 0.1 M Hydrochloric acid(HCl)
  • Glacial acetic acid
  • Tris base
  • 1 N NaOH
  • Reagent bottles
  • Glass pipette
  • Pipette pump
  • Boric acid

 

Procedure:-

 

Preparation of TE Buffer

  • Measure 10ml of 1M Tris-Cl buffer and 2ml of 0.5M EDTA solution.
  • Mix the solutions with distilled H2O and make up the volume to 1000ml using a graduated measuring cylinder.

 

Preparation of 10X TAE Buffer

  • Measure 10ml of 1M Tris-Cl buffer and 2ml of 0.5M EDTA.
  • Mix with distilled H2O and make up the volume to 1000ml using a graduated measuring cylinder.


Preparation of 5X TBE Buffer

  • Weigh out 54g of Tris base and 27.5g of Boric acid
  • Measure 20ml of 0.5M EDTA solution
  • Mix the contents with distilled H2O and make up the volume to 1000ml using a graduated measuring cylinder.

 

 

 

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