Flash content link: https://vlab.amrita.edu/repo/BIOTECH/MOL/Preparation_of_buffer_stocks/index.swf

 

Materials required

 

• Cotton                                                                                                                                                              
• Spatula                                                                                           
• magnetic stirrer                                                                       
• Butter paper   
• Forceps                                                                              
• Pipette rack                                                                                     
• 200ml beaker
• 1000ml beaker
• Distilled water
• measuring cylinders of 100ml , 1000ml(1L) ,  25ml and 10ml
• Glass rod
• Weighing balance 
• pH meter
• Wash bottle
• Tissue paper
• Dropper
• 0.1 M Hydrochloric acid(HCl)
• Glacial acetic acid
• Tris base
• 1 N NaOH
• Reagent bottles
• Glass pipette
• Pipette pump
• Boric acid

 

Procedure:-

 

Preparation of TE Buffer

• Measure 10ml of 1M Tris-Cl buffer and 2ml of 0.5M EDTA solution.
• Mix the solutions with distilled H₂O and make up the volume to 1000ml using a graduated measuring cylinder.

 

Preparation of 10X TAE Buffer

• Measure 10ml of 1M Tris-Cl buffer and 2ml of 0.5M EDTA.
• Mix with distilled H₂O and make up the volume to 1000ml using a graduated measuring cylinder.

Preparation of 5X TBE Buffer

• Weigh out 54g of Tris base and 27.5g of Boric acid
• Measure 20ml of 0.5M EDTA solution
• Mix the contents with distilled H₂O and make up the volume to 1000ml using a graduated measuring cylinder.

 

 

 

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