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Biotechnology and Biomedical Engineering
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Molecular Biology Virtual Lab II
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Polyacrylamide Gel Electrophoresis
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Polyacrylamide Gel Electrophoresis
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Theory
Procedure
Self Evaluation
Animation
Simulator
Assignment
Reference
Feedback
NPTEL Video
1)
Amino acids differ from one another because
They have different side chains
Some are found in nucleic acids
Some contain bound nucleotides
Some are resistant to hydrolysis
2)
Amino acids are linked together in proteins by______
Hydrogen bonds
Phosphodiester bonds
Peptide bonds
Hydrophobic interactions
3)
Which of the following are considered part of the secondary structure of a protein?
Alpha helix
Beta pleated sheet
Disulfide bonds
Both 1) and 2)
4)
SDS-PAGE has been used
To analyze different types of proteins in a biological sample
To characterize membrane proteins in their native conformation
To characterize the enzyme activities in the lysosome
To isolate nuclear DNA
5)
In an SDS-PAGE experiment, proteins are separated on the basis of their
Molecular weight
Charge to mass ratio
Different isoelectric points
Negatively charged side chain
6)
In an SDS-PAGE gel
Proteins are denatured by SDS
Proteins have the same charge to mass ratio
Smaller proteins migrate more rapidly through the gel
All the above
Cite this Simulator:
vlab.amrita.edu,. (2011). Polyacrylamide Gel Electrophoresis. Retrieved 25 April 2024, from vlab.amrita.edu/index.php?sub=3&brch=186&sim=319&cnt=3
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