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Western Blotting
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1)Western blotting is a technique that ..... 
  
2)Western blotting would be a useful technique to determine expression of a gene in a particular tissue. 
  
3)After incubation of membrane with primary antibody, which buffer is used for washing the membrane? 
  
4)The loading sample containing ....... 
  
5)The electrophoresis apparatus needs 
  
6)For verify protein transfer which stain is used? 
  
7)Primary antibody is diluted by using ........ 
  
8)Secondary antibody is diluted by using...... 
  
9)For the detection of protein in membrane which instrument is used? 
  
10)Assembly the immunblot sandwitch from cathode to anode. 1. Gel, 2. Filter paper, 3. Sponge, 4. PVDF membrane 
  

  


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