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Biotechnology and Biomedical Engineering
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Biochemistry Virtual Lab II
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Construction of Protein Standard Curve using Folin’s Lowry Method
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Construction of Protein Standard Curve using Folin’s Lowry Method
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
The role of folins ciocalteau reagent is :
Activation of copper to cuprous oxide
None of the above
Reduction of molybdate to molybdenum blue.
All the above.
2)
The role of alkaline copper reagent is :
Reduction of cupric ions to cuprous ions.
Oxidation of copper ions to cupric form.
Reduction of phosphomolybdate tungstate to heteromolybdenum blue.
None of the above.
3)
Phosphomolybdate is reduced by :
Tyrosine and tryptophan
Copper
Folins cioclateau reagent
All the above.
4)
Where is phosphomolybdatephosphotungstic acid found in the reaction:
Alkaline copper reagent
Folin’s ciocalteau reagent
Protein
Both a and b
5)
Why does the absorbance need to be measured at 650nm?
The folins lowry reagent has maximum absorption at 650nm.
The alkaline copper reagent will show its maximum absorption at 650nm.
The peptide bonds absorb at 650nm.
The absorption maximum of blue colour is at 650nm.
Cite this Simulator:
vlab.amrita.edu,. (2011). Construction of Protein Standard Curve using Folin’s Lowry Method. Retrieved 20 April 2024, from vlab.amrita.edu/index.php?sub=3&brch=64&sim=1087&cnt=3
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