Assignment:

1. Analyse the protein content in different samples using the above constructed protein standard curve.
2. Find out the specific activity of beta amylase enzyme .
3.  If 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein     when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample ?
4. If you mix two  unknown samples and repeat the lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is  used