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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab I
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ELISPOT Assay
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ELISPOT Assay
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
ELISPOT can be used to detect low frequencies of cells producing cytokine.
True
False
2)
Complete RPMI-10 medium contains RPMI-1640 with
15% FBS
10% FBS
1% FBS
20% FBS
3)
ELISPOT assays employ a technique very similar to the
Indirect ELISA
Direct ELISA
Sandwich ELISA
None of the above
4)
Cytokine secreted by activated cells is captured locally by the coated antibody on the high surface area PVDF membrane.
True
False
5)
The colored end product (spot) represents an individual cytokine-producing cell.
True
False
6)
Point out the applications of ELISPOT ASSAY:
It defines the vaccine efficiency
For diagnostic purposes
Investigate specific immune responses in various disease condition.
All of them
7)
The importance of ELISPOT Assay is that:
It measures the T-cell responses
Detect specific antibody secretion by B cells
Both of them
None of these
8)
The addition of substrate to the wells of microtiter plate and its incubation develops a colored product. which are the suitable substrates used in this assay:
3-Amino-9=ethylcarbazole/AEC
5-bromo-4-chloro-3-indolylphosphate-p-toluidine salt/BCIP
Nitro tetrazolium chloride /NBT
All of them
9)
Blocking solution is used in elispot assay for:
Remove unbound antibodies
Prevent the non-specific absorption of proteins
Production of colored products
None of them
10)
Which is the substrate that frequently used for alkaline phosphate enzyme:
BCIP
DAB
AEC
None of them
11)
Which is the protein inhibitor used for the additional control of stimulation of PBMC cells:
Cyloheximide
Cyclobenzene
Imatinib
Ritinovir
Cite this Simulator:
vlab.amrita.edu,. (2011). ELISPOT Assay. Retrieved 20 April 2024, from vlab.amrita.edu/index.php?sub=3&brch=69&sim=1098&cnt=3
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