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Purification of IgG Antibodies using Affinity Chromatography
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ASSIGNMENT

 

1.Define the following terms:

              A. Spacer Arm
              B. Fraction Collector
              C. Ligand Coupling

 

2.Explain the basic principle of Affinity Chromatography?

 

3. What are  the different types of elutions?

 

4 Explain the importance of Protein A in the purification of antibodies by affinity Chromatography?

 

5. What is Chaotropes?

 

 

 

 

 

 

 

 

 

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