Microbiology Virtual Lab I : Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab

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Microbiology Virtual Lab I

The study of microorganisms, which are unicellular or cell-cluster microscopic organisms. This includes eukaryotes such as fungi and, protists and prokaryotes. Viruses, though not strictly classed as living organisms, are also studied.

	Gram Stain Technique The Gram stain is a differential staining technique used to classify & categorize bacteria into two major groups: Gram positive and Gram negative, based on the differences of the chemical and physical properties of the cell wall.
	Aseptic Technique and the Transfer of Microorganisms Asepsis is the state of remaining free from pathogenic and contaminating microorganisms.This technique ensures a contaminant free environment while handling micro organisms.
	Streak Plate Method The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population.
	Motility Test To introduce and demonstrate the principle and experimental set up for determining the motility of microbes
	Catalase and Coagulase Test To introduce and demonstrate the principle and experimental set up for determining the microbe’s ability to detoxify hydrogen peroxide and /or to cause blood coagulation.
	Selective and Differential Media for Identifying Microorganisms To introduce the preparation and use of different selective and differential media
	Lecithinase Test To introduce and demonstrate the principle and experimental set up for determining the ability of microorganism to produce "Lecithinase" enzyme.
	Bacterial Growth Curve To study the different phases of growth of a bacterium by plotting a curve with time of growth on the X-axis and optical density on the Y-axis.
	Carbohydrate Fermentation Test To study the different carbohydrate utilization of bacteria using phenol red carbohydrate fermentation broth.
	Differential and Cytological Staining Techniques To study the staining of bacteria with special stains that help to reveal their morphology thereby enhancing the contrast using a bright field microscope.
	Antibiotic Susceptibility Testing To determine the susceptibility of a microbial species against different antibiotic agents.
	Methylene Blue Reductase Test Checking the quality of the given milk sample