Student's Assignment:

1.  What are the advantages and disadvantages of wet mount preparation?
2. How is motility associated with pathogenicity?
3.  Explain how SIM medium is used to detect motility?
4.  In addition to motility, for what other tests can SIM medium be used?
5.   What substrates are acted on in SIM medium in order for H₂S to be produced?
6.  Explain the function of the ferrous ammonium sulfate in SIM agar?
7.  Explain the characteristic behavior of P.vulgaris and E.aerogenes on SIM agar?
8.  A stool specimen of a patient with severe diarrhea was cultured in a series of specialized media for isolation of enteric organisms. The three isolates included species of Salmonella, Shigella and Escherichia. Explain how SIM agar test would be diagnostically significant?