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Catalase and Coagulase Test
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Student's Assignment:

 

  1. What is the substrate of the catalase reaction? Why are bubbles produced in a positive catalase test? Write a balanced equation for the degradation of H2O2 in the presence of catalase.
  2. Why will a false-positive catalase test result if the organisms are tested on a medium containing blood?
  3. Explain the toxic effect of oxygen on strict anaerobes.
  4. What is the importance of catalase to aerobic bacteria?
  5. Do anaerobic bacteria require catalase? Explain your answer.
  6. How does the enzyme coagulase function?
  7. False-positive coagulase tests have been reported for some bacteria that can metabolize citrate. Explain

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