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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab I
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Lecithinase Test
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Lecithinase Test
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
The medium commonly employed for lecithinase test is
Nutrient agar
Mannitol salt agar
Egg yolk agar
Mac Conkey agar
2)
Importance of Lecithinase test is
Differentiate between Staphylococcus aureus and Escherichia coli
Determine the ability of microorganisms to produce Lecithinase.
Identify bacteria which are capable of producing endoenzymes.
Both b and c.
3)
The opaque halo surrounding the bacterial colony when grown on egg yolk agar is due to
Break down of lecithin to phosphorylcholine and insoluble diglycerides.
Breakdown of phospholipids into phosphoglycerides.
Breakdown of starch into glucose by amylase.
None of the above.
4)
MYP agar is specifically used for identification of
Bacillus subtilis from food
Bacillus antracis from food
Bacillus megaterium from food
Bacillus cereus from food
5)
The lipoprotein component of egg yolk agar is
Diglycerides.
Phosphorylcholine.
Lecithovitellin.
Albumin.
6)
A causative agent of food poisoning in meat industry
Bacillus anthracis.
Clostridium tetani.
Pseudomonas aeroginosa.
Bacillus cereus.
7)
The pH indicator and antibiotic incorporated in MYP agar are
Bromothymol blue and polymyxin
Phenol red and polymyxin
Bromothymol blue and Streptomycin
Phenol red and Streptomycin
8)
Nagler’s reaction is commonly used for
Identification of Clostridium tetani
Identification of Clostridium perfringens
Identification of Bacillus cereus
Identification of Pseudomonas fluorescens
9)
Clostridium species are
Aerobic.
Obligate anaerobic.
Microaerophilic.
Facultative anaerobic.
10)
The dye in the Thioglycolate broth is
Phenyl red.
Methyl orange.
Resazurine.
Phenolphthalein
11)
A strong producer of Lecithinase is
Clostridium perfringens.
Clostridium difficile.
Bacillus subtilis.
All the above
12)
On incubation, Methylene blue strip in the anaerobic gas pak jar turn to colourless from blue. The reaction is due to
Liberation of Oxygen.
Liberation of Carbon dioxide.
Liberation of Sodium bicarbonate.
Addition of water.
Cite this Simulator:
vlab.amrita.edu,. (2011). Lecithinase Test. Retrieved 18 April 2024, from vlab.amrita.edu/index.php?sub=3&brch=73&sim=974&cnt=3
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