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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab II
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16S Ribosomal RNA Sequencing
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16S Ribosomal RNA Sequencing
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on ?
Size of the wells in the gel
Size of the separated DNA fragments
Number of DNA fragments in the gel
Volume of DNA sample loaded in the gel
2)
Role of Proteinase K in DNA extraction is
Precipitation of DNA
Deproteination of DNA
Solubilization of DNA
Maintenance of pH
3)
The technique that utilizes probes to detect specific DNA sequence
Northern blot
Western blot
Southern blot
Eastern blot
4)
Which of the following statements regarding the polymerase chain reaction is not true?
DNA polymerases from psychrophilic organisms is utilized
Amplify DNA of only a few base pairs up to a whole genome
Increase the amount of DNA in a sample
Has the potential of diagnosing an infection from a single copy of a gene
5)
Bacteria use restriction enzymes to
Synthesize RNA
Synthesize DNA
Destroy protein
Destroy foreign DNA
6)
Separation of DNA fragments using gel electrophoresis is applicable to all, except
Distinguish between different alleles of a gene
To purify specific DNA fragments
Identify DNA fragments for RFLP analysis
None of the above
7)
Which of the following is not a step in Southern blotting procedure?
Digestion of the DNA with restriction enzymes
Linking the DNA with DNA ligase
Separating the DNA fragments using gel electrophoresis
Transfer the DNA to a blot
8)
Lysozyme is important in DNA extraction because
It degrades the cell wall
It solubilizes the DNA
It maintains the pH of the buffer
All the above
9)
Principle behind the Southern blotting technique is
Molecular mass of DNA
Molecular mass of protein sequence
Similarities between the sequences of probe DNA and experimental DNA
Charge of biomolecules in the given sample
10)
A technique used to detect radioactive chemicals on X-ray film is
Electro blotting
Autoradiography
Electrophoresis
PCR
11)
In bacteria the gene that has proved to be the most informative for investigating evolutionary relatedness is
16S rRNA
5S rRNA
23S rRNA
All the above
12)
The āSā in 16 S rRNA represents
Rate of sublimation in seconds
Rate of sedimentation in Svedberg units
Rate of sedimentation per second
Rate of migration in seconds
Cite this Simulator:
vlab.amrita.edu,. (2012). 16S Ribosomal RNA Sequencing. Retrieved 24 April 2024, from vlab.amrita.edu/index.php?sub=3&brch=76&sim=1421&cnt=3
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