Procedure for accessing flash

Flash content link: https://vlab.amrita.edu/repo/BIOTECH/MIC/Litmus_Milk_Test/index.swf

 

Materials Required:

 

Cultures:

 

24-48 hourstryptic soy broth cultures of organisms

 

Media:

 

Litmus milk broth (pH-6.8)
Skim milk powder   100g
Litmus                       0.075g
Distilled water          1,000.0ml
 Autoclave at 12lb pressure for 15 minutes

 

Equipments:

 

• Bunsen burner
• Inoculating loop
• Test tube rack
• Marking pen

 

Procedure:

 

1. Using sterile technique, inoculate each experimental organism into its appropriately labeled tube by means of a loop inoculation. The last tube will serve as a control.
2. Incubate all cultures for 24 to 48 hours at 37˚C.

 

 

Limitations:

 

1. Litmus milk is a complex medium that can produce a diversity of results. Because of this, litmus milk can give quite unreliable results (i.e., the results often give negative results when something positive should happen). Thus, you would be advised to use litmus milk as a confirmatory test but not a definitive test (except as a last resort).
2. A clot formation is simply recorded as “clot” and cannot clearly differentiate between a clot and curd formation in this medium.

 

 

Help:

 

                    Unfortunately, this Virtual Lab requires Adobe Flash player.  Please see additional information if this does not work on your computer

• For Google Chrome - https://support.google.com/chrome/answer/6258784?co=GENIE.Platform%3DDesktop&hl=en

• For Microsoft Edge - https://support.microsoft.com/en-in/help/4532571/microsoft-edge-turn-on-flash 

• For Mozilla Firefox - https://support.mozilla.org/en-US/kb/install-flash-plugin-view-videos-animations-games

 

On GNU/Linux machines, please see appropriate online help. For example, 

 

• on Ubuntu - https://help.ubuntu.com/stable/ubuntu-help/net-install-flash.html.en 

• on Fedora - https://docs.fedoraproject.org/en-US/quick-docs/using-adobe-flash/