1. Text Download Section

Manual and theory.pdf

2. Serum 2D Gel Image Section

1. Serum_Control_coomassie_gel1.jpg
2. Serum_Control_coomassie_gel2.jpg
3. Serum_Control_coomassie_gel3.jpg
4. Serum_Case_coomassie_gel1.jpg
5. Serum_Case_coomassie_gel2.jpg
6. Serum_Case_coomassie_gel3.jpg

3. Video Download Section

1. Introduction to Proteomics
2. Introduction to Gel Based Proteomics
3. Serum Protein Extraction
4. Protein Quantification
5. First Dimension-Isoelectric Focusing
6. Second Dimension-SDS PAGE
7. Staining and Destaining
8. Data Analysis
   

4. Image Download Section

      HUMAN SERUM EXTRACTION

Withdrawal of Intravenous blood	Sonication of Serum Protein on ice	IgG, Albumin depeltion column packed with slurry	Depletion column	Vacutainer tube and	Acetone precipitated	Dried protein pellet

  

Whole blood in the	Blood Collection	Centrifugation on whole	Serum separated	Serum	Reconstruction of protein

 

 

QUANTIFICATION OF SERUM SAMPLE

Label tubes for assay	Add the sample to respective tube	Addition of Bradford	Color development after addition of dye	Read the O.D on Spectrophotometer

 

 

ISOELECTRIC FOCUSING

IEF apparatus	Rehydration of strip with protein sample	Addition of mineral oil	IEF Instrument	IEF Instrument with IEF tray

  

Placing wick on	Mounting electrodes	Mounting electrodes	Adding of mileral oil	IEF settings ready for focusing

 

 

SECOND DIMENSION SEPARATION-SDS PAGE

Small gel assembly	Small gel casting	Small gel assembly	Assembly of appartus	Glass plate assembly

  

Glass plate	Glass plate assembly	Mounting of molecular weight marker	Buffer tank	Serum proteins separated on 2DE stained with coomassie

 

 

STAINING AND DESTAINING

Partly stained gel	Destaining process	Destaining process	Imaging of the gel with scanner	Serum proteins separated on 2DE stained with coomassie	Silver stainning of serum protein

 

 

DATA ANALYSIS

Analysis Demo- 1	Analysis Demo- 2	Analysis Demo- 3	Analysis Demo- 4	Analysis Demo- 5	Analysis Demo- 6	Analysis Demo- 7

 

Analysis Demo-8	Analysis Demo-9	Analysis Demo-10	Analysis Demo-11	Analysis Demo-12	Analysis Demo-13	Analysis Demo-14

 

Analysis Demo-15	Analysis Demo-16	Analysis Demo-17	Analysis Demo-18	Analysis Demo-19	Analysis Demo-20	Analysis Demo-21

5. Assignment Download Section

A gel analysis is also carried out by us to show the analysis pattern and expected output. you may download the power point by clicking here:- Assignment_2.ppt. 

Assignment Slide-1	Assignment Slide-2	Assignment Slide-3	Assignment Slide-4	Assignment Slide-5	Assignment Slide-6

   

Assignment Slide-7	Assignment Slide-8	Assignment Slide-9	Assignment Slide-10	Assignment Slide-11	Assignment Slide-12

The quiz provided here helps the user to assess the level of understanding about the analysis. The quiz pr (Analysis_Quiz.ppt)  contains few questions based on analysis workflow. 

Quiz Slide-1	Quiz Slide-2	Quiz Slide-3	Quiz Slide-4

 Answers for the same is also provided here (Analysis_Answer_.ppt).

Answer Slide-1	Answer Slide-2	Answer Slide-3	Answer Slide-4	Answer Slide-5	Answer Slide-6	Answer Slide-7

  

Answer Slide-8	Answer Slide-9	Answer Slide-10	Answer Slide-11	Answer Slide-12	Answer Slide-13

6. Simulator Download Section

Animation of Two Dimensional Electrophoresis for Human Serum