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Biotechnology and Biomedical Engineering
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Molecular Biology Virtual Lab II
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Polymerase Chain Reaction (PCR)
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Polymerase Chain Reaction (PCR)
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Theory
Procedure
Self Evaluation
Simulator
Assignment
Reference
Feedback
NPTEL Video
1)
The number of amplified pieces of DNA equals -------- after five cycles of PCR
5
10
25
32
2)
Is it possible to use PCR to produce many copies of all DNA of one chromosome ?
yes, if the correct primers are available
yes,if the telomeres are present
No, PCR cannot copy DNA, only RNA
No, PCR copies short pieces of DNA only.
3)
PCR requires knowledge about parts of a target DNA sequence to be amplified. True or False?
True
False
4)
Which of the following is an advantage of single - primer PCR?
Less synthetic DNA is incorporated into the product
Unknown sequence adjacent to a known sequence can be amplified with a single primer
No Primer-dimer artifacts
5)
Which end of a PCR primer can be extended by polymerase?
The 1'end
The 2'end
The 3'end
The 4'end
6)
which nuclease activity does Taq polymerase NOT have?
5' to 3'
3' to 5'
7)
Which of the following significantly inhibits PCR?
12% PCR
Wooden toothpicks made of birch
0.2mM cresol red
8)
What is the minimum time required for denaturation in rapid- cycle PCR ?
One minute
30 seconds
Less than one second
9)
What is PCR good for ?
To check expression of a gene
To see if your gene is damaged
To make a few copies of your gene
To make a huge number of copies of a gene
Cite this Simulator:
vlab.amrita.edu,. (2011). Polymerase Chain Reaction (PCR). Retrieved 17 January 2025, from vlab.amrita.edu/?sub=3&brch=186&sim=321&cnt=3
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