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Polymerase Chain Reaction (PCR)




  1. What role do each of the following play in PCR - dNTPs, taq polymerase, primers?
  2. When the temperature rises to above 94oC there is chance of water evaporation. How is it prevented?
  3. What is the use of buffer in the reaction mixture?
  4.  A policeman had to investigate about a homicide of a woman. From the crime scene they got was a part of hair and semen from her body. What could be done to identify the murderer?


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