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Maintenance of Mamallian Cell Lines
1)Which is the indicator used in the cell culturing medium? 

2)Routinely used proteolytic enzyme to detach adherent cells from the substratum 

3)A student working in cell culture was not able to perform his experiments because of poor cell growth. can you help him by suggesting the possible reasons for the problem? 

4)How can you solve building up of toxic metabolites in the medium? 

5)Sub culturing of cells can be started at which phase of cell growth? 

6)Cells that do not attach normally to a substrate but remain in suspension with a spherical shape. 

7)An example for mammalian tissue culture media.


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