https://vlab.amrita.edu/repo/BIOTECH/ECO/Determination_of_PH_%20Waste_water_Sample/index.swf
Materials Required:
- Waste water collected from lake.
- Acetate buffer (pH = 4)
- Ammonium buffer (pH = 10)
- pH meter
- Tissue paper
Procedure:
- Collect water from the polluted lake and transfer it into a beaker.
- Switch on the pH meter.
- Remove electrodes from storage solution and rinse with water.
- Bloat with soaked tissue paper.
- Standardize the instrument with electrodes immersed in a buffer solution (Acetate buffer pH = 4).
- Rinse, bloat and dry the electrodes to each tie.
- Check the pH on the pH meter (pH = 7).
- Rinse, bloat and dry the electrodes.
- Standardize the instrument with electrodes immersed in a buffer solution (Ammonium buffer pH = 10).
- Rinse, bloat and dry the electrodes and check the pH on the pH meter (pH = 7).
- Dip the pH electrode in the beaker containing waste water to be tested. Note the pH.
- Replace the electrodes in the storage solution.