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Biotechnology and Biomedical Engineering
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Biological Image Analysis Virtual Lab
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Quantification of Stained Liver Cells
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Quantification of Stained Liver Cells
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Theory
Procedure
Self Evaluation
Simulator
Assignment
Reference
Feedback
1)
Which stain used for the quantification of liver tissue
DAPI
Methyl red
Trichrome
Osmium tetroxide
2)
Which command to view all three channels at the same time
Image-> Adjust-> Threshold
Image-> Stacks-> Make Montage
Image-> Type-> RGB stack
Image-> Type-> Make Montage
3)
ImageJ can be used to……………….
Measure the area of the selected portion
Measure differences in staining intensity of a tissue sample
Measure the % stained area of the selected portion.
All the above
4)
How to convert images into grey scale
Image-> type-> RGB stack
Image-> adjust-> color balance
Image-> color-> make composite
Process-> binary-> make binary
5)
Command to measure (quantify) the samples in a selected area is,
Ctrl+M
Analyse-> clear results
Analyze-> measure
Both a and c
All the above
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Cite this Simulator:
vlab.amrita.edu,. (2012). Quantification of Stained Liver Cells. Retrieved 25 January 2021, from vlab.amrita.edu/?sub=3&brch=278&sim=1466&cnt=3
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