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Biotechnology and Biomedical Engineering
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Biological Image Analysis Virtual Lab
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Counting of Total Fluorescence in a Cell
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Counting of Total Fluorescence in a Cell
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Theory
Procedure
Self Evaluation
Simulator
Assignment
Reference
Feedback
1)
To convert RGB image into a gray-scale image follow
Image --> Stacks --> RGB colour
Image --> Scale --> 8 bit
Image -->Type --> 8 bit
Adjust --> RGB colour -->8 bit
2)
The measurement (s) which are critical to analyze the total fluorescence per cell.
Standard Deviation
Integrated Density
Area Fraction
Perimeter
3)
A tool used to select a fluorescently stained cell for image analysis.
Rectangular selection tool
Wand tool
Free hand selection tool
Line tool
4)
Corrected Total Cell Fluorescence depends on
Integrated Density
Area of selected cell
Mean fluorescence of background readings
All the above
5)
Rectangular selection of non-fluorescent 1abeled sections in the imported image is important in image analysis because
It represents the area of the selected cell
It minimizes the errors in the fluorescent counting of a cell
It represents integrated density of the cell
It represents the Mean Grey value of the cell
Cite this Simulator:
vlab.amrita.edu,. (2012). Counting of Total Fluorescence in a Cell. Retrieved 10 December 2024, from vlab.amrita.edu/?sub=3&brch=278&sim=1474&cnt=3
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