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Biotechnology and Biomedical Engineering
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Biochemistry Virtual Lab I
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Separation of Amino Acids by Thin Layer Chromatography
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Separation of Amino Acids by Thin Layer Chromatography
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Theory
Procedure
Self Evaluation
Animation
Simulator
Assignment
Reference
Feedback
NPTEL Video
1)
Which of the following compounds is an amine:
CH
3
CN
CH
3
NO
2
CH
3
NH
2
None of the above
2)
Which among the following is an amino acid
Ethanolamine
Cytidine
Histidine
Thymine
3)
Which of the following is not an acid
H
3
PO
4
HCOOH
H
2
SO
4
All of these are acids
4)
A developed TLC plate has a solvent front of 6cm and a solute front of 3 cm . What is the Rf value of the compound.
3
2
0.5
0.2
5)
What is the maximum Rf value for any molecule in paper chromatography?
0.1
1.0
10
Infinity
6)
What does it mean when the Rf value of a molecule is 1?
The molecule has more affinity to mobile phase.
The molecule is more adsorbed onto stationary phase.
The interaction between molecule and mobile phase is weaker
None of the above.
7)
What does Rf value stands for:
Retention factor
Retardation factor
both Retention factor & Retardation factor
Relative factor
8)
when the thickness of adsorbent layer is increased, the Rf value will
remains the same.
decrease
Increase
None of the above.
Cite this Simulator:
vlab.amrita.edu,. (2011). Separation of Amino Acids by Thin Layer Chromatography. Retrieved 17 February 2025, from vlab.amrita.edu/?sub=3&brch=63&sim=154&cnt=3
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