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Quantitative Estimation of Amino Acids by Ninhydrin




  1. Perform the experiment using different α-aminoacids with different unknown concentrations.
  2. Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method.


3. Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of   same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.



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