Assignment:

1. Report the amount of precipitate as a function of the pH for the protein tried.
2. What would happen to the precipitation reaction if 0.2N H₂SO₄ is added instead of 0.2N HCl ?
3. Perform the isoelectric precipitation of egg albumin  and report it's isoelectric pH ?
4. A student while performing the casein precipitation using isoelectric pH, observed maximum precipitation at a pH=3.5. What could have happened during the experiment which have led to such an observation ?