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Isoelectric Precipitation of Proteins: Casein from Milk



  1. Report the amount of precipitate as a function of the pH for the protein tried.
  2. What would happen to the precipitation reaction if 0.2N H2SO4 is added instead of 0.2N HCl ?
  3. Perform the isoelectric precipitation of egg albumin  and report it's isoelectric pH ?
  4. A student while performing the casein precipitation using isoelectric pH, observed maximum precipitation at a pH=3.5. What could have happened during the experiment which have led to such an observation ?


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