Instructor content

The animation is self-illustrative. Instructors may choose to use the interactive version in class before detailing the protocol for column chromatography. Typically this approach suits any plant pigment column chromatography procedure.

Student Assignment

Click on the simulator window to load the animation. Participate in a full lab to go through the various stages to perform the chromatography.
You will have to click/move using the computer mouse to pass through various stages of the experiment.

Observe the size of beakers and volumes of compounds used. If you are unsure about the various steps, please repeat the experiment until you are familiar with all details.