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Biotechnology and Biomedical Engineering
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Biochemistry Virtual Lab II
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Gelatin Zymography
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Gelatin Zymography
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Zymography is an electrophoretic technique, based on ________ , that includes a substrate copolymerized with the polyacrylamide gel, for the detection of enzyme activity.
SDS PAGE
Protein
Blot analysis
None of the above
2)
The role of low salt collagenase buffer in zymography is:
Renaturation buffer
Denaturation
Activation buffer
None of the above
3)
The mechanism of activation of MMP-2 is referred to as:
Zinc dependent activation
Cysteine switch
Metalloprotease activation
All the above
4)
The role of Triton X-100 in zymography is:
Denaturation.
Removal of SDS from the gel.
Activation.
None of the above.
5)
The role of water saturated butanol is:
To prevent reaction with oxygen.
To speed up polymerization of gel.
To level off the resolving gel poured.
All the above.
6)
The white clear bands on the blue gel indicates:
Gelatinolytic activity
Metalloproteinase activity
Both a and b
None of the above
Cite this Simulator:
vlab.amrita.edu,. (2011). Gelatin Zymography. Retrieved 12 December 2024, from vlab.amrita.edu/?sub=3&brch=64&sim=700&cnt=3
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