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ELISPOT Assay
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1)ELISPOT can be used to detect low frequencies of cells producing cytokine. 
  
2)Complete RPMI-10 medium contains RPMI-1640 with 
  
3)ELISPOT assays employ a technique very similar to the 
  
4)Cytokine secreted by activated cells is captured locally by the coated antibody on the high surface area PVDF membrane. 
  
5)The colored end product (spot) represents an individual cytokine-producing cell. 
  
6)Point out the applications of ELISPOT ASSAY:
  
7)The importance of ELISPOT Assay is that:
  
8)The addition of substrate to the wells of microtiter plate and its incubation develops a colored product. which are the suitable substrates used in this assay:
  
9)Blocking solution is used in elispot assay for: 
  
10)Which is the substrate that frequently used for alkaline phosphate enzyme:
  
11)Which is the protein inhibitor used for the additional control of stimulation of PBMC cells: 
  

  


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