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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab I
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DIRECT Elisa
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DIRECT Elisa
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
NPTEL Video
1)
In enzyme-linked immunosorbent assay
Antibody is bound to an enzyme, which catalyzes the reaction
Substrates in the reaction is converted to a colored end-product
Antigen or antibody can be detected quantitatively with extremely sensitivity.
All the above
2)
The major disadvantage of Direct ELISA is
Cross-reactivity of secondary antibody is eliminated
Cross-reactivity might occur with the secondary antibody, resulting in nonspecific signal
Labeling primary antibodies for each specific ELISA system is time-consuming and expensive
Only one antibody and fewer steps are used
3)
Direct ELISA is used for the detection of
Soluble antibody
Soluble antigen
Complement
All of the above
4)
Which is not true for a blocking buffer solution ?
Binds to all potential sites of nonspecific interaction
Improves the sensitivity of an assay by reducing background signal
Improving the signal-to-noise ratio
Alters or obscures the epitope for antibody binding
5)
Washing step can be performed with a physiologic buffer such as tris-buffered saline (TBS) or phosphate-buffered saline (PBS)
True
False
Cite this Simulator:
vlab.amrita.edu,. (2011). DIRECT Elisa. Retrieved 17 February 2025, from vlab.amrita.edu/?sub=3&brch=69&sim=696&cnt=3
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