. .
1)In enzyme-linked immunosorbent assay 

2)The major disadvantage of Direct ELISA is 

3)Direct ELISA is used for the detection of 

4)Which is not true for a blocking buffer solution ? 

5)Washing step can be performed with a physiologic buffer such as tris-buffered saline (TBS) or phosphate-buffered saline (PBS) 


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