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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab II
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Fragmentation of IgG Using Papain
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Fragmentation of IgG Using Papain
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Which is the most abundant antibody present in serum?
IgG
IgE
IgA
IgD
2)
The enzyme Protease cleaves the IgG antibodies in hinge region and forms 2 fragments. Which of the following are the fragments formed by Papain?
F(ab)2 and Fc
2F(ab) and Fc
1F(ab) and 2Fc
None of the above
3)
Which is the chemical used for the further treatment of cleaved fragments of antibody?
70% Methanol
Mercaptoethanol
Both 1 & 2
None of the above
4)
Papain digested antibody is able to promote agglutination,percipitation,opsonization etc.
True
False
5)
Which is the chemical used for the further treatment of cleaved fragments of antibody?
70% Methanol
Mercaptoethanol
Both 1& 2
None of the above
6)
The enzyme Papain is present in which of the following fruit family?
Plum
Apricot
Papaya
Mango
7)
Which of the following are the applications of Papain?
Useful in Platelet Serology
Used in cell isolation procedure
Useful for structural studies of enzymes
All of them
8)
The applications of fragmented antibodies by papain are: a. Used in enzymatic synthesis of Amino acids b. Used for Antigen-Antibody Binding studies c. Used in Histochemical studies d. For Red Cell Serology
Both a and d
Both b and c
Only a
Only c
Cite this Simulator:
vlab.amrita.edu,. (2012). Fragmentation of IgG Using Papain. Retrieved 10 October 2024, from vlab.amrita.edu/?sub=3&brch=70&sim=1349&cnt=3
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