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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab I
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Carbohydrate Fermentation Test
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Carbohydrate Fermentation Test
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Theory
Procedure
Self Evaluation
Animation
Simulation
Assignment
Reference
Feedback
1)
The indicator used in the Phenol red carbohydrate fermentation test is
Brilliant green
Phenol red
Methylene blue
Phenolphthalein
2)
The importance of indicator in the Phenol red carbohydrate fermentation broth
Red in acidic condition
Yellow in acidic condition
Yellow in alkaline condition
Maintains the pH of the medium as 7.3
3)
The ability of microorganism to ferment carbohydrate mainly depends on
The concentration of the carbohydrate
The time of incubation of the inoculated carbohydrate broth
The enzyme systems of the bacteria
None of the above
4)
Fermentation will result in ----------------
ATP production
Alkaline end products
Acidic end products with or without gas production
All the above
5)
After the incubation of the culture, the phenol red broth remains unchanged. This is because
The carbohydrate is fermented to organic acids
The bacteria cannot utilize the given carbohydrate
The pH of the medium is acidic
Lack of incubation
6)
In the acidic pH the phenol red changes its color from
Pink to red
Yellow to red
Red to yellow
Pink to yellow
7)
Acid and gas production during fermentation indicates-------------
The organism uses the given carbohydrate and produced organic acids and gas
The organism is anaerobic and did not use the carbohydrate
Increases the pH of the media
The peptones in the medium are degraded
8)
Phenol red carbohydrate fermentation broth is
Enriched media
Selective media
General purpose media
None of the above
9)
The production of gas during fermentation can be determined by
Yellow color of the broth
Checking the pH of the broth
Checking the bubbles in the inverted durham tubes
Both b and c
10)
The concentration of sugar added in the Phenol red Carbohydrate fermentation test is
10 % to 15%
0.5% to 1%
0.1% to 0.5%
0.5% to 10%
11)
Carbohydrates can be sterilized at 121 degree Celcius
True
False
12)
The application of high temperature for sugar sterilization will result in ---------
Browning effect of sugar
Caramelization
Hydrolyze sugar
Both a and b
Cite this Simulator:
vlab.amrita.edu,. (2011). Carbohydrate Fermentation Test. Retrieved 17 February 2025, from vlab.amrita.edu/?sub=3&brch=73&sim=1139&cnt=3
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