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Differential and Cytological Staining Techniques


Student's Assignment:


  1. Explain the importance of steam during the acid-fast staining procedure.
  2. Discuss the advantages of differential staining procedures over the simple staining techniques.
  3. Explain the function of copper sulfate in capsule staining.
  4. Negrosine stain is applied over bacterial smear in negative staining. Discuss.
  5. Explain why thick or dense smears less likely to provide a good smear preparation for microscopic evaluation.
  6. Basic stains are more effective for bacterial staining than acidic dyes. Discuss.

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