Procedure for accessing flash
Animation link: https://vlab.amrita.edu/repo/BIOTECH/MIC/Methylene_Blue_Reductase_Test/index.swf
Materials Required:
- Milk samples to be analyzed
- Screw-cap test tubes
- Test tube rack
- Pipettes(10 ml and 1ml)
- Water bath (37o C)
- Bunsen Burner
Procedure:
- Transfer 10 ml of each milk sample into appropriately labeled test tube.
- Add 1 ml of redox indicator, methylene blue to each test tube containing milk sample.
- Tighten the test tube mouth with stoppers. Gently invert the tubes at about four or five times to ensure proper mixing of the methylene blue solution.
- Keep the tubes in the water bath at 37 o C
- Note the incubation time. That is, the time elapsed for the color to turn whitish appearance.
- Stabilize the tubes for 5 minutes.
Result Interpretation
- Reduction within 30 minutes: Very poor quality
- Reduction occurring between 30 minutes and 2 hours : Poor quality
- Reduction occurring between 2 and 6 hours: Fair quality
- Reduction occurring between 6 and 8 hours: Good quality
- Not reduced in 8 hours: Excellent quality
Help:
Unfortunately, this Virtual Lab requires Adobe Flash player. Please see additional information if this does not work on your computer
• For Google Chrome - https://support.google.com/chrome/answer/6258784?co=GENIE.Platform%3DDesktop&hl=en
• For Microsoft Edge - https://support.microsoft.com/en-in/help/4532571/microsoft-edge-turn-on-flash
• For Mozilla Firefox - https://support.mozilla.org/en-US/kb/install-flash-plugin-view-videos-animations-games
On GNU/Linux machines, please see appropriate online help. For example,
• on Ubuntu - https://help.ubuntu.com/stable/ubuntu-help/net-install-flash.html.en
• on Fedora - https://docs.fedoraproject.org/en-US/quick-docs/using-adobe-flash/