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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab I
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Selective and Differential Media for Identifying Microorganisms
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Selective and Differential Media for Identifying Microorganisms
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Theory
Procedure
Self Evaluation
Animation
Simulator
Assignment
Reference
Feedback
1)
Phenylethyl alcohol agar is
Differential
Selective
Enriched
Nutrient
2)
Blood agar is used to cultivate and isolate many types of bacteria, but it is best known for its role in differentiating the members of Streptococcus
True
False
3)
Percentage of sodium chloride in mannitol salt agar is_______
9%
7.5%
75%
8.5%
4)
The development of yellow halos around the bacterial growth on a mannitol salt agar plate is evidence of a pathogenic species of Staphylococcus
True
False
5)
On EMB agar, if ________ is grown it will give a distinctive metallic green sheen appearance
Escherichia coli
Pseudomonas aeruginosa
Helicobacter pylori
Klebsiella pneumonia
6)
EMB agar is a blend of two stains, eosin and ________ in the ratio of 6:1
Crystal violet
Methylene blue
Phenyl red
Phenolphthalein
7)
Hektoen enteric agar a selective and differential agar primarily used to isolate________ and ___________
Salmonella, Shigella
Escherichia, Salmonella
Proteus, Klebsiella
Staphylococcus, Shigella
8)
The indicator in Mannitol Salt agar is neutral red
True
False
9)
MacConkey’s Agar contains_________ that inhibits most Gram-positive bacteria, except Enterococcus and some species of Staphylococcus
Cholesterol
Bile salts
Neutral red
Lactose
10)
MacConkey agar is a differential medium containing lactose, bile salts, neutral red and crystal violet
True
False
11)
Which of the following inhibits the growth of Gram positive bacteria?
Eosin methylene blue agar
MacConkey’s Agar
Hektoen Enteric (HE) Agar
All of the above
12)
Phenylethyl alcohol agar is a selective medium that allows growth of Gram positive organisms
True
False
13)
Mannitol salt agar plates are used for the differentiation of the members of the genus?
Staphylococcus
Streptococcus
Enterobacter
Enterococcus
14)
In EMB agar, one of the indicators used is Methylene Blue. Which is the other?
Phenol red
Crystal violet
Eosin
Ebony
15)
Phenylethyl alcohol agar supports the growth of__________
Escherichia coli
Salmonella typhimurium
Staphylococcus aureus
Pseudomonas aeruginosa
Cite this Simulator:
vlab.amrita.edu,. (2011). Selective and Differential Media for Identifying Microorganisms. Retrieved 23 April 2024, from vlab.amrita.edu/?sub=3&brch=73&sim=720&cnt=3
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