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Isolation and Identification of Two Bacterial Unknowns


Student's Assignment:


  1. Explain the principle behind catalase and coagulase test?
  2. You are provided with a mixed culture of organism. Provide a flow chart to show different procedure involved in the identification process.
  3. Explain a rapid biochemical test for the identification of Staphylococcus aureus?
  4. Explain the principle of nitrate reduction test?
  5. Explain the principle of nitrate reduction test?
  6. While performing the gram stain technique, you noticed that the culture contains gram negative rods and gram negative cocci. Explain in detail about your further proceedings of the experiment?
  7. Explain the following:

    1. Alpha hemolysis
    2. Beta hemolysis
    3. Gamma hemolysis.
  8. A bacterium grown in the starch agar plate is suspected to have the enzyme amylase. How will you confirm the presence of amylase in the medium?
  9. Explain the different biochemical tests of bacteria that rely on the fermentation of carbohydrates?
  10. Escherichia coli has the ability to grow on the EMB agar plate, but Staphylococcus aureus cannot. Explain the reason?



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