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16S Ribosomal RNA Sequencing




  1. Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for analyzing a particular gene sequence? Discuss.
  2. Discuss the importance of adding radioactively labeled molecules during 16S ribosomal RNA sequencing process of a bacterial genome.
  3. Construct a flow chart elucidating the steps in identification of 16S ribosomal RNA of a given sample.
  4. Nucleic acids usually migrate towards the negative electrodes. Validate the statement.
  5. Electro blotting techniques are routinely used in diagnostic microbiology for detection of a specific DNA sequence in the given bacterial culture. Explain.
  6. Specify the role of different chemicals employed in DNA extraction.

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