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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab II
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Triple Sugar Iron Agar
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Triple Sugar Iron Agar
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
TSI slants contain
Glucose (1.0%), Lactose (0.1%) and Sucrose (1.0%)
Glucose (1.0%), Lactose (1.0%) and Sucrose (1.0%)
Glucose (0.1%), Lactose (1.0%) and Sucrose (1.0%)
Glucose (0.1%), Lactose (1.0%) and Sucrose (1.0%)
2)
The blackening of the slant and the breaks in the agar indicate
carbon dioxide and hydrogen sulfide production respectively
Hydrogen sulfide and carbon dioxide respectively
No utilization of carbohydrates
None of the above
3)
The visible black precipitate in TSI slant after incubation is
Ferrous sulfate
Copper sulfate
Ferrous sulfide
Lead sulfide
4)
Which of the following is true about TSI medium?
It contains glucose, sucrose and lactose
It contains the pH indicator phenol red
it contains thiosulfate and ferrous sulfate
All the above
5)
Alkaline slant (red) and acid butt (yellow) with or without gas production (breaks in the agar slant) is indicative of
No carbohydrate fermentation
Lactose/Sucrose fermentation
Glucose fermentation
Hydrogen Sulphide production
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Cite this Simulator:
vlab.amrita.edu,. (2011). Triple Sugar Iron Agar. Retrieved 6 March 2021, from vlab.amrita.edu/?sub=3&brch=76&sim=216&cnt=3
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