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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab II
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Bacteriophage Plaque Assay for Phage Titer
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Bacteriophage Plaque Assay for Phage Titer
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Theory
Procedure
Self Evaluation
Animation
Simulator
Assignment
Reference
Feedback
1)
A bacteriophage is ____________
a virus that infects a bacterium
a bacterium that infects a virus
a bacterium
a virus
2)
For a valid pahge count, the number of plaques per plate should be
More than 300
Less than 30
In between 30-300
None of the above
3)
A plaque assay detects the number of __________
Bacterial auxotrophs
Dental cavities
Bacterial viruses
Bacterial prototrophs
4)
Forty-eight (48) plaques were counted in a plate when 0.1 ml of bacteriophage-infected bacteria from 10¯⁶ dilution (10 ml culture) was plated. The original bacteriophage density was:
4.8 X 10⁶
48 X 10¯⁶
4.8X 10¯ ⁷
48 X 10⁷
5)
In the plaque assay, the plaques on the agar plate are areas where:
Infected bacteria have lysed
Uninfected bacteria are growing
Phages are growing
The phages have died
6)
If the plaque assay serial dilution factor is too low:
There will be too few bacteria cells lysed to accurately count
The plaques will fuse, lysing the entire culture
Nothing will happen
None of the bacteria will be lysed
Cite this Simulator:
vlab.amrita.edu,. (2011). Bacteriophage Plaque Assay for Phage Titer. Retrieved 26 April 2024, from vlab.amrita.edu/?sub=3&brch=76&sim=719&cnt=3
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