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Biotechnology and Biomedical Engineering
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Molecular Biology Virtual Lab I
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Maintenance and Storage of DH5alpha E.coli cells
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Maintenance and Storage of DH5alpha E.coli cells
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Usually glycerol is added at a concentration of
15%
22%
30%
10%
2)
Which one is the cryoprotective agent that enters the cell and protects the intracellular environment of the organism?
Lactose
Polyvinylpyrrolidone
Glycerol
Polyglycol
3)
Storage of microorganisms in liquid nitrogen at temperature
-196 ºC
-110 ºC
-169 ºC
109 ºC
4)
The cryoprotective agents which are used for lyophilization
Dextran
Skim milk
Sucrose
Both 2 and 3
5)
Which temperature is used in short term preservation methods
-20 ºC
-60 ºC
-30 ºC
20 ºC
Cite this Simulator:
vlab.amrita.edu,. (2011). Maintenance and Storage of DH5alpha E.coli cells. Retrieved 11 December 2024, from vlab.amrita.edu/?sub=3&brch=77&sim=1095&cnt=3
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