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Plasmid Isolation (Mini prep)


Instructors content


Animation is self instructive. Instructors may choose to use the interactive version in class before detailing the protocol of agarose gel electrophoresis


Students Assignment


Click on the simulator window to load the animation. Participate in a full lab to go through the various stages to perform isolation of plasmid by alkaline lysis. You will have to click or move using the computer mouse to pass through various stages of experiment. You can choose any type of bacterial cells containing plasmid for isolating the plasmid. Presence of plasmid can be detected by using agarose gel electrophoresis.




  1. Classify plasmid based on their confirmation?
  2. What are the different steps in the isolation of plasmid? 


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