Preparation of Equilibrated Phenol Through this experiment you will able to understand the preparation of equilibrated phenol and its role in the biological/molecular experiments. | |
Isolation of RNA To understand isolation of RNA, the most critical step in performing many fundamental molecular biology experiments. | |
Polyacrylamide Gel Electrophoresis PAGE (Polyacrylamide Gel Electrophoresis) , is the most widely used analytical method to resolve separate components of a protein mixture based on their size. | |
Ligation ( Using T4 DNA Ligase) Ligation ( Using T4 DNA Ligase) | |
Polymerase Chain Reaction (PCR) Polymerase chain reaction is one of the technologies that not only made a tremendous impact on the scientific community, but also affected many aspects of our everyday lives. | |
Electroblotting To familiarize the user with the technique of Electroblotting | |
Plating of the Bacteriophage To demonstrate the ability of bacteriophage to replicate inside a susceptible host cell and to determine the concentration of phage particles in a suspension. | |
Plasmid Curing Plasmids are extrachromasomally replicating molecules of DNA present in bacteria.Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin ,acridine orange,SDS, etc Various methods involv | |
Extraction of Bacteriophage DNA from Large Scale Cultures Using Proteinase K and SDS To extract genomic DNA from The bacteriophage In a lysate. | |
Preparation of stocks of bacteriophage lambda by plate lysis and elution To prepare the stocks of bacteriophage in a laboratory. |
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