Assignment

1. How western blot does works and explain some of its possible application?
2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why was the SDS necessary for running the gel? Why was it necessary for  the transfer to the PVDF membrane?
3. The first stage of western blotting involves separating proteins using SDS- PAGE. On what basis does this technique separate proteins?
4. How can you test the efficiency of transfer of the protein from the gel to the PVDF  membrane?
5. What does the primary antibody recognize and what does the secondary antibody recognize? Which antibody has HRP conjugated to it?